實(shí)驗(yàn)流程: 細(xì)胞或組織蛋白樣品制備—蛋白定量—電泳(Electrophoresis)—轉(zhuǎn)膜(Transfer)—封閉(Blocking)—一抗孵育(Primary antibody incubation)—二抗孵育(Secondary antibody inucubation)—蛋白檢測(Detection of proteins)—膜的重復(fù)利用(Membrane recovery)—數(shù)據(jù)分析。